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Updated: Aug 02, 2026
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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Blood Banking (Immunohematology) | 15-20% | - Transfusion reactions and adverse events - Blood components and storage - Antibody detection and identification - Blood group systems and antigens - Compatibility testing |
| Clinical Chemistry | 20-25% | - Endocrinology and therapeutic drug monitoring - Carbohydrates, lipids, proteins, and enzymes - Electrolytes, acid-base balance, and blood gases - Toxicology and liver/kidney function tests |
| Hematology | 20-25% | - White blood cell morphology and diseases - Red blood cell physiology and disorders - Hemostasis and coagulation testing - Routine and automated hematology procedures |
| Immunology and Serology | 5-10% | - Autoimmune and immunodeficiency disorders - Immunologic testing methodologies - Immune system principles and components - Infectious disease serology |
| Microbiology | 15-20% | - Identification of pathogenic microorganisms - Mycology, parasitology, and virology - Specimen collection and culture techniques - Bacteriology and antimicrobial susceptibility |
| Laboratory Operations | 5-10% | - Laboratory safety and regulatory compliance - Pre-analytical, analytical, and post-analytical processes - Quality control and quality assurance - Laboratory mathematics and instrumentation |
| Urinalysis and Other Body Fluids | 5-10% | - Cerebrospinal, serous, and synovial fluids - Physical, chemical, and microscopic analysis - Semen and amniotic fluid testing - Urine formation and composition |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. The organisms in this image are demonstrating a gram-variable phenomenon. For the most part, the organisms are staining primarily gram-negative, however, on the tips of some of the rods, there is a gram-positive staining morphology. This can be defined as gram-variable.
Which of the following best describes the organisms seen in this illustration:
A) gram-variable
B) gram-positive
C) acid-fast
D) gram-negative
2. Electrophoresis uses an electric field to separate molecules based on differences in net electrical charge. The rate of electrophoretic migration is also dependent on the ionic radius of the molecule, the viscosity of the solution through which it is migrating, the electrical field strength, temperature, and the type of supporting medium used.
Electrophoretic separation fundamentally relies on:
A) Net charge differences of molecules
B) Shape variations of molecules
C) Size differences of molecules
D) Concentration differences of molecules
3. Parathyroid hormone regulates serum calcium by acting on bone, kidney, and intestines while regulating phosphate by stimulating the intestines and the kidneys, enhancing absorption and reabsorption respectively.
Chem
The parathyroid hormone is important in the regulation of:
A) acid and alkaline phosphatase
B) iodine and bromine
C) zinc and magnesium
D) calcium and phosphate
4. What is the CORRECT blood-to-anticoagulant ratio for coagulations tests?
A) 10:1
B) 5:1
C) 9:1
D) 4:1
5. The results of this PT and aPTT are in normal range. These results can be reported and are not indicative of the need to: order a mixing study or request a redraw.
You have just performed stat PT and aPTT tests on your coagulation instrument. Your results are as follows:
PT = 12 seconds (normal range 10-13 seconds)
aPTT = 24 seconds (normal range 21-34 seconds)
What would be your next step?
A) Request a redraw of the specimen
B) Report the results
C) Perform a mixing study
Solutions:
| Question # 1 Answer: A | Question # 2 Answer: C | Question # 3 Answer: D | Question # 4 Answer: C | Question # 5 Answer: B |
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